| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166649 |
| Chromosome: | chromosome 2 |
| Location: | 3579656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095134 | (1 of 2) 2.1.1.9 - Thiol S-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGATTGCGTGGACGTGTGCTTGCGCGAT |
| Internal bar code: | TGGCATGCGCGTTCTTGTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 65 |
| LEAP-Seq percent confirming: | 34.375 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACGAGGAGAGTTGGGTG |
| Suggested primer 2: | ATGTCAGTTGCTTGGGGAAC |