| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166651 |
| Chromosome: | chromosome 10 |
| Location: | 5007254 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455451 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGCAAGGATGGAGGATAAGGTGACTGG |
| Internal bar code: | GAACGAAGATAGGGTAAGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.7597 |
| LEAP-Seq n confirming: | 2491 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACTGCCTACTCGCTGCT |
| Suggested primer 2: | TACATGCCGTTGTCGTTTGT |