| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166655 |
| Chromosome: | chromosome 4 |
| Location: | 2496850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221950 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGTATGGAACGAAGCAGGTCGCGAAAT |
| Internal bar code: | ATACCACTAGACCTAGCATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 99.5671 |
| LEAP-Seq n confirming: | 690 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTTCCCGTGAGAGCTGG |
| Suggested primer 2: | CTGGGCATAAGGACCTTGAA |