Insertion junction: LMJ.RY0402.166656_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.166656
Chromosome:chromosome 8
Location:249891
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g358554 (1 of 14) 3.1.3.16//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCAACAAGCACACCCCCATCACTACCT
Internal bar code:TTCGCGGAGCATTGCGGGACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:826
LEAP-Seq percent confirming:73.6253
LEAP-Seq n confirming:6842
LEAP-Seq n nonconfirming:2451
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAGAACTACCGTCCGCTC
Suggested primer 2:GTTTTGCAGGTCCATGGTTT