Insertion junction: LMJ.RY0402.166656_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.166656
Chromosome:chromosome 13
Location:471182
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g564900 MRP3 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; ABC transporter, multidrug resistance associated protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGGCTTCTTGCCTTTTCATCCGCTGTTC
Internal bar code:TGGGACATTGTAAATCGCTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:807
LEAP-Seq percent confirming:86.1111
LEAP-Seq n confirming:930
LEAP-Seq n nonconfirming:150
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTAGCAAGAAGGGACAAG
Suggested primer 2:TTTCGTGCTTGTCTGAAACG