| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166665 |
| Chromosome: | chromosome 5 |
| Location: | 2171605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234645 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATAGCTGATGTGGTTTTAAAAAGGAAG |
| Internal bar code: | CCTACTGAGAGGTCAAAATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 83.8647 |
| LEAP-Seq n confirming: | 2999 |
| LEAP-Seq n nonconfirming: | 577 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGGTTGGGTGTTAGAGA |
| Suggested primer 2: | CAAACTCCTGAGCCACCTTC |