| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166699 |
| Chromosome: | chromosome 4 |
| Location: | 2132610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAGGCACGGCCGCGCCTCTCGCCCCGC |
| Internal bar code: | GAGGTATACTATAGGGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1083 |
| LEAP-Seq percent confirming: | 92.7961 |
| LEAP-Seq n confirming: | 2499 |
| LEAP-Seq n nonconfirming: | 194 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGACACCAGGAACTCGG |
| Suggested primer 2: | GCATCATACAACGCATACCG |