| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166703 |
| Chromosome: | chromosome 13 |
| Location: | 1513854 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572750 | AMX3 | (1 of 1) 1.4.3.22 - Diamine oxidase / SSAO; Copper amine oxidase, amiloride-sensitive | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTCATGCGCTCCACCATTGTGGTGAGC |
| Internal bar code: | GTTCGGGGAGTCCCTTGTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1069 |
| LEAP-Seq percent confirming: | 87.2432 |
| LEAP-Seq n confirming: | 4459 |
| LEAP-Seq n nonconfirming: | 652 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTTAGAAGGCACTGGC |
| Suggested primer 2: | AGTGAACCCAGGTGTAACGC |