Insertion junction: LMJ.RY0402.166730_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.166730
Chromosome:chromosome 14
Location:1868331
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g620600 PHC2,FAP202 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGCCTTGGAGCAGCAGTAATCGGTGCA
Internal bar code:GAGCCGTTGTGTTGAAGACCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1007
LEAP-Seq percent confirming:99.8418
LEAP-Seq n confirming:3787
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGATGGAGTAGGGGAACA
Suggested primer 2:TTCACAGATTTGTCGCTTCG