| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166732 |
| Chromosome: | chromosome 7 |
| Location: | 1413400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322900 | (1 of 1) K13806 - sn1-specific diacylglycerol lipase (DAGL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGGGTGCGCGATTTGGGATAGAGACGG |
| Internal bar code: | ATAGGATACTAGCTTATGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 99.3705 |
| LEAP-Seq n confirming: | 3315 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCATCAGAAAGGGGAGGA |
| Suggested primer 2: | GTGCTCCAGCAGTACGACAA |