| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166750 |
| Chromosome: | chromosome 13 |
| Location: | 1924867 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576150 | APN1 | (1 of 1) PTHR11707//PTHR11707:SF3 - L-ASPARAGINASE // SUBFAMILY NOT NAMED; L-asparaginase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGAGAGCCACCTTTCTTGTCTACCTGTC |
| Internal bar code: | CACCCGGAGAGGCCACGTATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 99.0521 |
| LEAP-Seq n confirming: | 209 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCATGAATGTGACCGCT |
| Suggested primer 2: | ACAAATCAGAGGCGGACATC |