| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166755 |
| Chromosome: | chromosome 12 |
| Location: | 6535703 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539000 | ECT1 | (1 of 1) 2.7.7.14 - Ethanolamine-phosphate cytidylyltransferase / Phosphorylethanolamine transferase; CDP-Ethanolamine synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGTGGGTGTTAGAACTGTTCGCACTGA |
| Internal bar code: | GAGCTGACGATGAGGGTACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 99.6729 |
| LEAP-Seq n confirming: | 1219 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCATCATGTCGAAGCAG |
| Suggested primer 2: | ACGGTTGGTTGAGAGTTTGG |