| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166783 |
| Chromosome: | chromosome 11 |
| Location: | 2053704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468650 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACAAAATGTGGCATGCGGGATATCAGGT |
| Internal bar code: | CGAAGGTCGTATTCAATTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 99.5818 |
| LEAP-Seq n confirming: | 8096 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACACAGCACTGACTTGC |
| Suggested primer 2: | TCTCAACAAGCCATTTGCAC |