Insertion junction: LMJ.RY0402.166844_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.166844
Chromosome:chromosome 15
Location:166863
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g635067 (1 of 3) PTHR30570//PTHR30570:SF0 - PERIPLASMIC PHOSPHATE BINDING COMPONENT OF PHOSPHATE ABC TRANSPORTER // PHOSPHATE-BINDING PROTEIN PSTS intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGGGGGTGCGGAGTGGAGGAGGCGGAG
Internal bar code:GGGTACCTGTACTGTAGTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:441
LEAP-Seq percent confirming:99.436
LEAP-Seq n confirming:10579
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTCACACCCTGTCTGTT
Suggested primer 2:COULD_NOT_FIND_PRIMER