| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166852 |
| Chromosome: | chromosome 10 |
| Location: | 5894676 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461900 | AKR8 | Aldo/keto reductase; (1 of 2) PTHR11732:SF255 - ARYL-ALCOHOL DEHYDROGENASE YPL088W-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGATGTCAGCGGCGCACTCATCCGAGA |
| Internal bar code: | ATCTGTCCATACAGGTAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 13.5025 |
| LEAP-Seq n confirming: | 2682 |
| LEAP-Seq n nonconfirming: | 17181 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTGAGTACGAGAAGGTG |
| Suggested primer 2: | GCAAAGCGATTCCTGCTTAC |