| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.166852 |
| Chromosome: | chromosome 10 |
| Location: | 5894769 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461900 | AKR8 | Aldo/keto reductase; (1 of 2) PTHR11732:SF255 - ARYL-ALCOHOL DEHYDROGENASE YPL088W-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTGTCTTGTTGCTTGCATGGTCAAACC |
| Internal bar code: | AGCCTTATCTTATTTCTACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1267 |
| LEAP-Seq percent confirming: | 98.0265 |
| LEAP-Seq n confirming: | 3626 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAGCGATTCCTGCTTAC |
| Suggested primer 2: | TGCGTGAGTACGAGAAGGTG |