Insertion junction: LMJ.RY0402.166872_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.166872
Chromosome:chromosome 13
Location:683281
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g566350 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGATGGCATCATGACAGGCAACACCACC
Internal bar code:TGCTGGGCGGGTTCGTGTAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:661
LEAP-Seq percent confirming:97.861
LEAP-Seq n confirming:2928
LEAP-Seq n nonconfirming:64
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTGTCTGATTGCGTCGTC
Suggested primer 2:CGCAGGTTGCCATGTAGATA