Insertion junction: LMJ.RY0402.166873_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.166873
Chromosome:chromosome 1
Location:6258170
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g044800 DGAT1,PFL1 (1 of 1) 2.3.1.54 - Formate C-acetyltransferase / Pyruvate formate-lyase; Pyruvate-formate lyase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCTCTTCCGCCCACTTCCACAATTCGC
Internal bar code:TGACTGGAGTGGAGGGGTATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:3703
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:TATCTGCCGTTGTGCTTCTG
Suggested primer 2:GATTCGGACCTTGTCATCGT