Insertion junction: LMJ.RY0402.166907_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.166907
Chromosome:chromosome 4
Location:3496858
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g227950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGATGACTGATGAGTGGCAGGGTGGGGCG
Internal bar code:AACCATTTCTCGTGAATGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:176
LEAP-Seq percent confirming:99.4681
LEAP-Seq n confirming:1309
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAACAGGCTCATGACGAA
Suggested primer 2:GTCCAACTACCGCCATCTGT