| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166912 |
| Chromosome: | chromosome 3 |
| Location: | 3553764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTAATATGGCTTAGGACTGGCTTGGCGC |
| Internal bar code: | GCGGGTTTACTGGGCAGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 88.3495 |
| LEAP-Seq n confirming: | 182 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAAGTTCGAGAAGCCAG |
| Suggested primer 2: | TCTATCACCGTGACCGTGTG |