| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166921 |
| Chromosome: | chromosome 11 |
| Location: | 2455367 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475450 | (1 of 1) K11863 - Ataxin-3 (ATXN3, MJD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCTTGCCTTTACTCCCTGCTACTTGCG |
| Internal bar code: | GATGCCAGCGAGCCTGGTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 98.1968 |
| LEAP-Seq n confirming: | 2505 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTGGGGACAGAATTGC |
| Suggested primer 2: | GCGAGTTTGAGGATGAGGAC |