Insertion junction: LMJ.RY0402.166945_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.166945
Chromosome:chromosome 9
Location:7413236
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g413250 GT90-15,GT90F15 GT90 family protein 15; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGTCCTCCCCGAGTCTCCACAGCTCATA
Internal bar code:TGATGCCGGCAGCATAGGTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:182
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCTTGTGCTCTCTGTCA
Suggested primer 2:ACACCCCTGCTGGATAGTTG