Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.166961 |
Chromosome: | chromosome_16 |
Location: | 4937359 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g685450 | DHC8 | Flagellar inner arm dynein heavy chain, dynein e (monomeric) | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCCTCTGTTAGTGCGACCTCCGCGCCATG |
Internal bar code: | ACGTGTCAGCGTAACTGTGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 805 |
LEAP-Seq percent confirming: | 98.8924 |
LEAP-Seq n confirming: | 625 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTGCCTTGTGGACTCCTAA |
Suggested primer 2: | CTCCTCCATGTCCACATCCT |