| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.166969 |
| Chromosome: | chromosome 6 |
| Location: | 8175703 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305302 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCATGCATACCGCGAGCGCGGTGCTGGC |
| Internal bar code: | GGGCACTCCCTCGACCAGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 98.3574 |
| LEAP-Seq n confirming: | 1976 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAACAAAACCTGTGCCA |
| Suggested primer 2: | ACATCCAATAGCAGGTTGCC |