| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166972 |
| Chromosome: | chromosome 2 |
| Location: | 790163 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078858 | PSN1 | Presenilin protease; (1 of 1) K04505 - presenilin 1 (PSEN1, PS1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGAGAGTGTGTGTTGCAGGGGGGCACGA |
| Internal bar code: | ACGTGGCGCGATTGTCGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 78.7879 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCAGAGGGAATCAGCAC |
| Suggested primer 2: | TAGGGGTTTGCGTACAGGAC |