Insertion junction: LMJ.RY0402.166975_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.166975
Chromosome:chromosome 10
Location:3463842
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g444850 CAV7 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCACCACCCACACCACCACGGCGGGG
Internal bar code:CGTAAGAGATCCGATGGTGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:552
LEAP-Seq percent confirming:98.9691
LEAP-Seq n confirming:192
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGAACATCTCATCCTCTG
Suggested primer 2:ACTGTGCATGTTCAAACCCA