Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.166996 |
Chromosome: | chromosome 7 |
Location: | 388104 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g314833 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCGCACAAACCAAAACTGCACTTATCC |
Internal bar code: | GCTCACTCGATCGGACAATCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 618 |
LEAP-Seq percent confirming: | 97.7927 |
LEAP-Seq n confirming: | 1019 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACCTTTTGCAGCTCACC |
Suggested primer 2: | TGTGCAACCGCATTAGAGAG |