| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167004 |
| Chromosome: | chromosome 8 |
| Location: | 938891 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGCCGCCCGCCATGCCCGCCTCGCGC |
| Internal bar code: | GCGGGGAGAAAGGGGAAATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 51.8782 |
| LEAP-Seq n confirming: | 2776 |
| LEAP-Seq n nonconfirming: | 2575 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGCTATGTGGTGATGCC |
| Suggested primer 2: | CAGCAGTAAGGTGTGCAGGA |