| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167010 |
| Chromosome: | chromosome 17 |
| Location: | 540667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699600 | SBP2,SEBP2 | (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; putative Sugar bisphosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTACTGCCACAGCCAATCATCAACAG |
| Internal bar code: | GGCGTGGTGGTCGCCCCTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 88.3333 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTTTTTCGTTCATTTGT |
| Suggested primer 2: | GCAAGAACTCCTGAACCTCG |