| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167020 |
| Chromosome: | chromosome_16 |
| Location: | 1944063 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g656300 | DPR1 | Dihydrodipicolinate reductase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTTCTAACCAAACCTACACCCACATCACA |
| Internal bar code: | AGCGTCTAGTTATTGAGCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 99.2155 |
| LEAP-Seq n confirming: | 7715 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGTGTGAAAGCACAGGC |
| Suggested primer 2: | ATGCTGCACCTCCTCTCACT |