Insertion junction: LMJ.RY0402.167041_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.167041
Chromosome:chromosome_6
Location:2166314
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g265950 DHC3 Flagellar inner arm dynein heavy chain, novel minor type dynein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCCTCGCCGCTGTCGCTGTACGACGTGCAC
Internal bar code:GCGTTAGGGCTCGGATGTTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:363
LEAP-Seq percent confirming:27.5016
LEAP-Seq n confirming:2600
LEAP-Seq n nonconfirming:6854
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGTTCACCGACTCCTC
Suggested primer 2:ACCCTCCACCAGGAACATCT