Insertion junction: LMJ.RY0402.167041_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.167041
Chromosome:chromosome 6
Location:2166321
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g265950 DHC3 Axonemal Inner Arm Dynein Heavy Chain 3, pseudogene; (1 of 2) PTHR10676:SF136 - DYNEIN HEAVY CHAIN 6, AXONEMAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCAGCTGCCAGCAGCTGCTCCGCCGTC
Internal bar code:GAAAACGACAAGGTGCGGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:41
LEAP-Seq percent confirming:99.2453
LEAP-Seq n confirming:263
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGTTCACCGACTCCTC
Suggested primer 2:ACCCTCCACCAGGAACATCT