| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167044 |
| Chromosome: | chromosome 12 |
| Location: | 1279052 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489200 | (1 of 1) PTHR10794//PTHR10794:SF28 - ABHYDROLASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCGGCAAAGCGATACCAAATTTTGCCC |
| Internal bar code: | GCGTTTCTAGTTCCGGCAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 8.73016 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 805 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGTACCAGGGAAAGGCT |
| Suggested primer 2: | CTGGAACTGCGTTCTGTTCA |