Insertion junction: LMJ.RY0402.167068_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.167068
Chromosome:chromosome 12
Location:5207446
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g527918 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGCCCAAACGCCGCATGACCGTTACGTC
Internal bar code:CACCTCCAAGGGCCCCGAATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:98.5885
LEAP-Seq n confirming:9569
LEAP-Seq n nonconfirming:137
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTCTGAATATGGTCCCCT
Suggested primer 2:CTCTGCAGGACACAAGTCCA