| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167071 |
| Chromosome: | chromosome 6 |
| Location: | 3204162 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275950 | FAP395 | Flagellar Associated Protein 395 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAAGAAGCCGACCGAGTACGAGTACAGC |
| Internal bar code: | TTTGCCTGTATGCATCGCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 90.8795 |
| LEAP-Seq n confirming: | 2232 |
| LEAP-Seq n nonconfirming: | 224 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCATGTTGTCGATGTTC |
| Suggested primer 2: | GATGCAGATGCAGATGATGC |