| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167129 |
| Chromosome: | chromosome 17 |
| Location: | 6983703 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746247 | (1 of 1) K11430 - histone-lysine N-methyltransferase EZH2 (EZH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCGGGGTGGTGGTCGGGCTGGTCGGG |
| Internal bar code: | CGCTATTCGTTCGATAGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 63.1203 |
| LEAP-Seq n confirming: | 7505 |
| LEAP-Seq n nonconfirming: | 4385 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGACCCCTTGACAGACAT |
| Suggested primer 2: | ACACTGCAAATACAACCCCC |