Insertion junction: LMJ.RY0402.167134_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.167134
Chromosome:chromosome 8
Location:1874913
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g366900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGCTCGTGCACACCTACCGTTTGCTCAT
Internal bar code:CTTTTTGATTTTGACCCTACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:98.1519
LEAP-Seq n confirming:1434
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCATCAATGTCGTCACAGCA
Suggested primer 2:ACGCGGAGGGATACTAGGTT