Insertion junction: LMJ.RY0402.167160_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.167160
Chromosome:chromosome 2
Location:2743605
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g093850 (1 of 1) IPR000104//IPR000595//IPR001611//IPR003591//IPR018490 - Antifreeze protein, type I // Cyclic nucleotide-binding domain // Leucine-rich repeat // Leucine-rich repeat, typical subtype // Cyclic nucleotide-binding-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTATGCTAGATGCGCGTGACATTCCGCTG
Internal bar code:ATAAGTTCTGCACTAGATACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:48
LEAP-Seq percent confirming:2.66046
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:2927
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCCATGAGCAAAATCATA
Suggested primer 2:AGGTGCTAAACATGCATCCC