| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167167 |
| Chromosome: | chromosome 1 |
| Location: | 5851127 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041800 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGAACCTTGCCGTCCAGCAGGCGGG |
| Internal bar code: | TTGTCCGACCCGAAGGTTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 98.1928 |
| LEAP-Seq n confirming: | 1956 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGGCGAACTCTGAGAC |
| Suggested primer 2: | GGTGGTCATGATTGTTGCAG |