Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.167174 |
Chromosome: | chromosome_3 |
Location: | 2412216 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g159016 | antisense | intron|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAAGTGCCACCACATCGTTTTTCGGGCAGC |
Internal bar code: | CATCGATGCCGGATTGGATTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 678 |
LEAP-Seq percent confirming: | 99.6194 |
LEAP-Seq n confirming: | 8115 |
LEAP-Seq n nonconfirming: | 31 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTCGCTCTCATAACGATGC |
Suggested primer 2: | GTCAAGTCCGATTGCTGGAT |