| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167174 |
| Chromosome: | chromosome_3 |
| Location: | 5432421 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g185050 | FAP53 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGCGTATTGGTATAGCCACCCACCTCTC |
| Internal bar code: | GGTCGCTACTAGCTGTGCCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 98.9028 |
| LEAP-Seq n confirming: | 631 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAGGCGTTCATCCAGAG |
| Suggested primer 2: | CACATGCTCACCAATCGAAC |