| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167177 |
| Chromosome: | chromosome 3 |
| Location: | 4700523 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177750 | MAE10 | (1 of 5) PTHR11206:SF94 - DNA-DAMAGE-INDUCIBLE PROTEIN F; related to EDS5, enhanced disease susceptibility gene | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCTCTTTTTGGTACTGCAGCACGCCTG |
| Internal bar code: | GCCATCAAGAACTTAGCTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 98.4674 |
| LEAP-Seq n confirming: | 3855 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGGCATACAATGCTG |
| Suggested primer 2: | CTTGCACTCCATGCAACAGT |