| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167190 |
| Chromosome: | chromosome 15 |
| Location: | 1282035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641650 | HLM22 | (1 of 4) PTHR10887:SF331 - HELICASE MOV10L1-RELATED; Histone-lysine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGCTATGGCTTCATCAGCCTCTTTTGT |
| Internal bar code: | CTCTTCGATAATTCCGTAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 99.2255 |
| LEAP-Seq n confirming: | 2306 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCCCCCTGCAGTAAATA |
| Suggested primer 2: | TACGGTGACGGTTACGATGA |