| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167218 |
| Chromosome: | chromosome 7 |
| Location: | 3647455 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337150 | RFC2 | (1 of 1) PTHR11669:SF5 - REPLICATION FACTOR C SUBUNIT 2; DNA replication factor C complex subunit 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGTCAGTGGCGGGTCCGCGTGCACGG |
| Internal bar code: | AAAATTGATGTGTTGGTCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 99.5378 |
| LEAP-Seq n confirming: | 1292 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTACCACTTCCCTCCA |
| Suggested primer 2: | ACTGATGTCACGCAGCAGTC |