| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167231 |
| Chromosome: | chromosome 12 |
| Location: | 6278678 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537150 | (1 of 20) IPR008972 - Cupredoxin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCTAGGCATGTGCGGAAGACTTGCTTC |
| Internal bar code: | TGGGAGTTGCTTTACCTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 99.9057 |
| LEAP-Seq n confirming: | 1059 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTACCTTGTGCAACTCA |
| Suggested primer 2: | CCCAGTTCCTGACTGGTTGT |