| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167267 |
| Chromosome: | chromosome 10 |
| Location: | 3796486 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446850 | (1 of 10) PF02373 - JmjC domain, hydroxylase (JmjC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGAGTGTCAGCACTGAATGGCCGGGAT |
| Internal bar code: | ATTACCCTATGTGGCAGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 99.5496 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGAAGCCAGAGCAACA |
| Suggested primer 2: | AGAGGGTCATGTGAATTGGC |