Insertion junction: LMJ.RY0402.167270_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.167270
Chromosome:chromosome 1
Location:5144091
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g035800 IRK2 (1 of 2) PTHR11767//PTHR11767:SF47 - INWARD RECTIFIER POTASSIUM CHANNEL // SUBFAMILY NOT NAMED; Inwardly-rectifying potassium channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGGCGTTGCGTGTCGGGGTTGAGGCTC
Internal bar code:TTGGTTAGTAGAACCCCTATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:687
LEAP-Seq percent confirming:98.7484
LEAP-Seq n confirming:4576
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTTCTTGACCCCATCACG
Suggested primer 2:ACTGGACCGACATCTTCCAC