| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167301 |
| Chromosome: | chromosome 4 |
| Location: | 2194655 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219925 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGATGGTACGCTTAGGGCGGCGGAGG |
| Internal bar code: | TCGCAGAGGGTAAGGCGGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 47.8811 |
| LEAP-Seq n confirming: | 870 |
| LEAP-Seq n nonconfirming: | 947 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTATGCTTTTTGCGTGT |
| Suggested primer 2: | GCTTTAAGGAACGAAATGCG |