| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167332 |
| Chromosome: | chromosome 10 |
| Location: | 2748240 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438600 | (1 of 1) PF09072 - Translation machinery associated TMA7 (TMA7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGTCGCTGTCGTCGTAGTCCTTGTCC |
| Internal bar code: | GACGCACTATCGAGAGCACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 99.6258 |
| LEAP-Seq n confirming: | 5059 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTAGCGGTGCAGATGAAA |
| Suggested primer 2: | CCAGCACACAATTCATCACC |