| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167333 |
| Chromosome: | chromosome 16 |
| Location: | 265858 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694403 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCAATAATCTATTGGCTGTAGCTGCCA |
| Internal bar code: | CTCGGTAACGAACGGGTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 92.8303 |
| LEAP-Seq n confirming: | 74941 |
| LEAP-Seq n nonconfirming: | 5788 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACCGCTGAGTAAATGAA |
| Suggested primer 2: | AGTGCGGTCAGCAGTAGGTT |